Peggy 試薬と消耗品

Peggy試薬と消耗品はPeggy専用に製造されており、Compassソフトウェアver 1.8以上と互換ファームウェアとあわせて使用してください。

Size Separation Assay Kits

Part # Description
CBS-01-01 Rabbit (12-180 kDa) Size Separation Master Kit for Sally or Peggy
Core Kit for Peggy or Sally, 5 boxes of Capillaries (96 capillaries per box), Size Separation Kit for Peggy or Sally, Size Detection Kit-Rabbit for Peggy or Sally, and Simple Western Standard 1 (MW ladder spans 12-180 kDa). Reagents for 480 Simple Western data points. Ships in 2 parts.
CBS-02-01 Mouse (12-180 kDa) Size Separation Master Kit for Sally or Peggy
Core Kit for Peggy or Sally, 5 boxes of Capillaries (96 capillaries per box), Size Separation Kit for Sally or Peggy, Size Detection Kit-Mouse for Peggy or Sally, and Simple Western Standard 1 (MW ladder spans 12-180 kDa). Reagents for 480 Simple Western data points. Ships in 2 parts.
CBS301 Core Kit for Sally or Peggy
Includes Matrix Removal Buffer, Wash Buffer, Running Buffer, Luminol-S, Peroxide, Running Buffer cup, Wash Buffer cup, Matrix Removal cup, 5 Manifold Cleaning Sponges and 5 Plates. Reagents for 480 Simple Western data points. Ships in 2 parts.
CBS301S1 Size Separation Kit for Sally or Peggy
Includes Separation Matrix, Stacking Matrix, 2 Fluorescent Standard vials, Standard Resuspension Buffer, Sample Buffer and 8 DTT vials. Reagents for 480 Simple Western data points. Ships in 2 parts.
CBS301RD Size Detection Kit-Rabbit for Sally or Peggy
Goat-Anti-Rabbit Secondary HRP Conjugate (ready-to-use reagent) and Antibody Diluent. Reagents for 480 Simple Western data points.
CBS301MD Size Detection Kit-Mouse for Sally or Peggy
Goat-Anti-Mouse Secondary HRP Conjugate (ready-to-use reagent) and Antibody Diluent. Reagents for 480 Simple Western data points.

Charge Separation Assay Kits

Part # Description
CBS2001 XDR Charge Separation Master Kit for Peggy Sue, Peggy or NanoPro 1000
Includes 5 boxes of capillaries, five 384-well plates with lids, Anolyte, Catholyte, Wash Concentrate, Luminol, and Peroxide XDR and manifold cleaning sponges.
CBS404 HeLa Lysate Controls Kit - Bicine/CHAPS Buffer for Charge Assays
Includes 5 vials each of low phospho and high phospho HeLa lysate, lysed in Bicine/CHAPS Lysis Buffer (5 µL/vial). Ships overnight on dry ice. Upon receipt, store components at 4°C.
CBS403 Lysis Kit - Bicine/CHAPS Buffer for Charge Assays
Includes Bicine/CHAPS Lysis Buffer, Aqueous Inhibitor, DMSO Inhibitor Mix, and Cell Wash Buffer. Ships overnight on dry ice. Upon receipt, store components at 4°C.
CBS401 Lysis Kit - RIPA Buffer for Charge Assays
Includes RIPA Buffer, Aqueous Inhibitor, DMSO Inhibitor Mix and Cell Wash Buffer. Ships overnight on dry ice. Upon receipt, store components at 4°C.
041-126 Amplified Rabbit Secondary Antibody Detection Kit
Includes SA-HRP conjugate (50 µL), Secondary Goat-Anti-Rabbit-Biotin Conjugate (50 µL); Antibody Diluent included. REAGENT FOR PEGGY OR NANOPRO 1000 USERS ONLY. ProteinSimple strongly recommends use of the Antibody Diluent for dilution of primary and secondary antibodies as it has been proven superior to any other buffer system tested. Ships overnight at room temperature. Upon receipt, store at 4°C.
041-127 Amplified Mouse Secondary Antibody Detection Kit
Includes SA-HRP conjugate (50 µL), Secondary Goat-Anti-Mouse-Biotin Conjugate (50 µL); Antibody Diluent included. REAGENT FOR PEGGY OR NANOPRO 1000 USERS ONLY. ProteinSimple strongly recommends use of the Antibody Diluent for dilution of primary and secondary antibodies as it has been proven superior to any other buffer system tested. Ships overnight at room temperature. Upon receipt, store at 4°C.
PS-T003 Peggy System Charge Training Kit
Includes a XDR Charge Separation Master Kit for Peggy Sue, Peggy or NanoPro 1000 and additional reagents

Individual Reagents

Part # Description
CBS201STD1 Simple Western Size Standard 1 for Sally, Peggy and Simon
Biotinylated Ladder 1 (MW ladder spans 12-180 kDa), Ladder Resuspension Buffer and Streptavidin-HRP. Reagents for 480 Simple Western data points.
041-695 Single Use FL standard
Single Use FL standard-strip of 8 tubes (for use with Simple Western Size Standard 1 MW Ladder 12-180 kDa)
041-247 Separation Matrix
Separation matrix 1 (2.5 mL)
041-248 Stacking Matrix
Stacking matrix (1.5 mL)
041-265 Simon, Sally, Peggy Sample Buffer
Simon, Sally, Peggy 10X Sample Buffer (400 µL)
041-808 Antibody Diluent Plus
Antibody Diluent Plus for size assay (10 mL) for Simon, Sally or Peggy. Ships overnight at room temperature. Antibody Diluent is recommended for dilution of primary and secondary antibodies. Upon receipt, store at 4°C.
041-505 Running Buffer for Sally or Peggy
Running Buffer (140 mL)
041-504 Matrix Removal Buffer for Sally or Peggy
Matrix Removal Buffer (140 mL)
041-586 Wash Buffer for Sally or Peggy
Wash Buffer (170 mL)
043-311 Luminol-S
Luminol-S (2 mL) for use on Jess™, Abby™, Wes™, Peggy Sue and Sally Sue systems.
043-379 Peroxide
Peroxide (2 mL) for use on Jess™, Abby™, Wes™, Peggy Sue and Sally Sue systems.
041-468 HeLa Lysate Control - Bicine/CHAPS Buffer for Sally, Peggy or Simon
Includes 1 vial of HeLa lysate, lysed in Bicine/CHAPS Lysis Buffer (35 µL/vial)
040-655 Goat-Anti-Mouse-HRP Secondary Antibody
HRP-conjugated Goat-Anti-Mouse Secondary Antibody (50 µL); Antibody Diluent included. ProteinSimple strongly recommends use of the Antibody Diluent for dilution of primary and secondary antibodies as it has been proven superior to any other buffer system tested. Ships overnight at room temperature. Upon receipt, store at 4°C.
040-656 Goat-Anti-Rabbit Secondary Antibody
HRP-conjugated Goat-Anti-Rabbit Secondary Antibody (50 µL); Antibody Diluent included. ProteinSimple strongly recommends use of the Antibody Diluent for dilution of primary and secondary antibodies as it has been proven superior to any other buffer system tested. Ships overnight at room temperature. Upon receipt, store at 4°C.
041-081 Goat-Anti-Rabbit HUX HRP Secondary Antibody
HRP-Conjugated Goat-Anti-Rabbit Secondary Antibody cross-absorbed for minimal cross-reactivity against human antigens (50 µL); Antibody Diluent included. ProteinSimple strongly recommends use of the Antibody Diluent for dilution of primary and secondary antibodies as it has been proven superior to any other buffer system tested. Ships overnight at room temperature. Upon receipt, store at 4°C.
040-474 Pan Erk Primary Antibody
Pan Erk Primary Antibody, 1X stock (1 mL)
040-477 Phospho-Erk 1/2 Primary Antibody
Phospho-Erk 1/2 Primary Antibody, 1X stock (1 mL); Ships overnight at room temperature. Upon receipt, store at 4°C.
040-644 pI Standard Ladder 1
pI Standard Ladder 1: 4.0, 4.9, 6.0, 6.4, 7.3 (175 µL); Ships overnight at room temperature. Upon receipt, store at 4°C.
040-645 pI Standard Ladder 2
pI Standard Ladder 2: 4.2, 4.9, 6.0, 6.4, 7.0 (175 µL); Ships overnight at room temperature. Upon receipt, store at 4°C.
040-646 pI Standard Ladder 3
pI Standard Ladder 3: 4.9, 6.0, 6.4, 7.0, 7.3 (175 µL); Ships overnight at room temperature. Upon receipt, store at 4°C.
040-647 pI Standard Ladder 4
pI Standard Ladder 4: 4.9, 5.5, 6.0 (175 µL); Ships overnight at room temperature. Upon receipt, store at 4°C.
040-024 pI Standard, 4.0
pI Standard, 4.0 (50 µL); Ships overnight at room temperature. Upon receipt, store at 4°C.
040-025 pI Standard, 4.2
pI Standard, 4.2 (50 µL); Ships overnight at room temperature. Upon receipt, store at 4°C.
040-026 pI Standard, 4.4
pI Standard, 4.4 (50 µL); Ships overnight at room temperature. Upon receipt, store at 4°C.
040-027 pI Standard, 4.9
pI Standard, 4.9 (50 µL); Ships overnight at room temperature. Upon receipt, store at 4°C.
040-028 pI Standard, 5.5
pI Standard, 5.5 (50 µL); Ships overnight at room temperature. Upon receipt, store at 4°C.
040-029 pI Standard, 6.0
pI Standard, 6.0 (50 µL); Ships overnight at room temperature. Upon receipt, store at 4°C.
040-030 pI Standard, 6.4
pI Standard, 6.4 (50 µL); Ships overnight at room temperature. Upon receipt, store at 4°C.
040-031 pI Standard, 7.0
pI Standard, 7.0 (50 µL); Ships overnight at room temperature. Upon receipt, store at 4°C.
040-032 pI Standard, 7.3
pI Standard, 7.3 (50 µL); Ships overnight at room temperature. Upon receipt, store at 4°C.
041-036 pI Standard, 8.4
pI Standard, 8.4 (50 µL); Ships overnight at room temperature. Upon receipt, store at 4°C.
040-790 pI Standard, 9.7
pI Standard, 9.7 (50 µL); Ships overnight at room temperature. Upon receipt, store at 4°C.
040-967 Premix G2, ampholyte-free separation gradient
Premix G2, ampholyte-free separation gradient (1.5 mL); Ships overnight at room temperature. Upon receipt, store at 4°C and in the dark.
040-968 Premix G2, pH 3–10 separation gradient
Premix G2, pH 3–10 separation gradient (1.5 mL); Ships overnight at room temperature. Upon receipt, store at 4°C and in the dark.
040-970 Premix G2, pH 4–7 (nested) separation gradient
Premix G2, pH 4–7 (nested) separation gradient, pH 2-4 plug (1.5 mL); Ships overnight at room temperature. Upon receipt, store at 4°C and in the dark.
040-969 Premix G2, pH 4–9 separation gradient
Premix G2, pH 4–9 separation gradient (1.5 mL); Ships overnight at room temperature. Upon receipt, store at 4°C and in the dark.
040-971 Premix G2, pH 5–6 separation gradient
Premix G2, pH 5–6 separation gradient (1.5 mL); Ships overnight at room temperature. Upon receipt, store at 4°C and in the dark.
040-972 Premix G2, pH 5–8 (nested) separation gradient, pH 2–4 plug
Premix G2, pH 5–8 (nested) separation gradient, pH 2–4 plug (1.5 mL); Ships overnight at room temperature. Upon receipt, store at 4°C and in the dark.
040-973 Premix G2, Pharmalyte pH 5–8 separation gradient
Premix G2, Pharmalyte pH 5–8 separation gradient (1.5 mL); Ships overnight at room temperature. Upon receipt, store at 4°C and in the dark.
040-974 Premix G2, Servalyt pH 5–8 separation gradient
Premix G2, Servalyt pH 5–8 separation gradient (1.5 mL); Ships overnight at room temperature. Upon receipt, store at 4°C and in the dark.
040-337 Anolyte Refill
Anolyte Refill (200 mL)
040-338 Catholyte Refill
Catholyte Refill (200 mL)
041-108 Wash Concentrate Refill for Peggy or NanoPro 1000
Wash Concentrate Refill (200 mL). REAGENT FOR PEGGY or NANOPRO 1000 USERS ONLY. Ships overnight at room temperature. Upon receipt, store at 4°C.
040-652 Luminol
Luminol (2 mL); Ships overnight at room temperature. Upon receipt, store at 4°C.
040-483 RIPA Lysis Buffer
RIPA Lysis Buffer (10 mL); Ships overnight at room temperature. Upon receipt, store at 4°C.
040-764 Bicine/CHAPS Lysis Buffer and Sample Diluent
Bicine/CHAPS Lysis Buffer and Sample Diluent (10 mL); Ships overnight at room temperature. Upon receipt, store at 4°C.
040-510 DMSO Inhibitor Mix
DMSO Inhibitor Mix, 50X (200 µL); Ships overnight at room temperature. Upon receipt, store at 4°C.
040-482 Aqueous Inhibitor Mix
Aqueous Inhibitor Mix, 25X (400 µL); Ships overnight on dry ice. Upon receipt, store at -20°C.
040-649 Sample Diluent
Sample Diluent (3 mL); Ships overnight at room temperature. Upon receipt, store at 4°C.
040-309 Antibody Diluent
Antibody Diluent (10 mL)
041-428 DTT for Peggy or Sally
Single-use DTT - strip of 8 tubes. For Peggy or Sally.
041-595 Instrument Cup (set of 3)
Includes set of 3 instrument cups
041-270 Streptavidin-HRP for Simon, Sally, or Peggy
Streptavidin-HRP Conjugate (120 µl) (ready-to-use reagent) for use with Simon, Peggy and Sally
041-606 Pipetting Guide
Pipetting guide for Sally, Peggy, Sally Sue or Peggy Sue Size Assay

Consumables

Part # Description
MR700 Capillaries - Size Separation for Peggy/Sally (1 pack)
Peggy/Sally Capillaries for size assays (1 box of 96 capillaries)
MR701 Capillaries - Size Separation for Peggy/Sally (5 pack)
Peggy/Sally Capillaries for size assays (5 boxes of 96 capillaries)
CBS700 Capillaries-Charge Separation (1 pack) for Peggy or NanoPro 1000
Peggy, Peggy Sue and NanoPro 1000 Capillaries for charge assays (1 box of 96 capillaries)
CBS701 Capillaries-Charge Separation (5 pack) for Peggy or NanoPro 1000
Peggy, Peggy Sue and NanoPro 1000 Capillaries for charge assays (5 boxes of 96 capillaries)
040-663 Assay Plate/Lid Kit (5-Pack) for Peggy/Sally, Peggy Sue/Sally Sue, or NanoPro 1000
Includes five 384-well plates and lids. * Must be ordered with p/n 041-528.
041-528 Sponge Pack for Peggy/Sally, Peggy Sue/Sally Sue, or NanoPro 1000
Includes 6 manifold cleaning sponges. * Must be ordered with p/n 040-663.