Simple Western technical library

For a list of peer-reviewed articles our products have been cited in, check out the publications page.

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Application Briefs

Application Brief 1001: p-EGFR NanoPro Assay   
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Application Brief 1002: AKT NanoPro Assay   
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Application Brief 1003: PAK2 NanoPro Assay   
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Application Brief 1004: ALAS1 Loading Control NanoPro Assay   
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Application Brief 1005: GSK3α NanoPro Assay   
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Application Brief 1006: GSK3β NanoPro Assay   
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Application Brief 1007: Thioredoxin1 Loading Control NanoPro Assay   
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Application Brief 1008: Hsp70 NanoPro Assay   
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Application Brief 1009: Hsp70 Loading Control NanoPro Assay   
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Application Brief 1010: Caspase 3 NanoPro Assay   
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Application Brief 1011: β-2-Microglobulin Loading Control NanoPro Assay   
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Application Brief 1012: p-STAT3 NanoPro Assay   
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Application Brief 1013: p-STAT5 NanoPro Assay   
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Application Brief 1014: PLCγ1 NanoPro Assay   
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Application Brief 1015: ERK1/2 NanoPro Assay   
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Application Brief 1016: MEK1 NanoPro Assay   
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Application Brief 1017: MEK2 NanoPro Assay   
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Application Brief 1018: Src NanoPro Assay   
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Application Brief 1019: p-JNK NanoPro Assay   
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Application Brief 1020: phospho-p27/Kip1 NanoPro Assay   
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Application Brief 1021: 4E-BP1 NanoPro Assay   
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Application Brief 1022: 4E-BP2 NanoPro Assay   
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Application Brief 1023: Crk-L NanoPro Assay   
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Application Brief 1024: c-Myc Epitope Tag NanoPro Assay   
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Application Brief 1025: FLAG Epitope Tag NanoPro Assay   
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Application Brief 1026: HA Epitope Tag NanoPro Assay   
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Application Brief 1027: ASNS NanoPro Assay   
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Application Brief 1028: LDH NanoPro Assay   
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Application Brief 1029: cIAP1 NanoPro Assay   
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Application Brief 1030: GFP NanoPro Assay   
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Application Brief 1031: eEF2 NanoPro Assay   
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Application Brief 1032: Mfn1 NanoPro Assay   
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Application Brief 1033: Mfn2 NanoPro Assay   
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Application Brief 1034: Survivin NanoPro Assay   
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Application Brief 1035: Phospho-p38 NanoPro Assay   
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Application Brief 1036: GAPDH Loading Control NanoPro Assay   
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Application Brief 1037: PTEN NanoPro Assay   
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Application Brief 1038: Histone NanoPro Assay   
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Application Brief 1039: Acetylation NanoPro Assay   
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Application Notes

Using the Simple Western Total Protein Assay to Normalize Immunoassay Data in the Same Run   
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Accelerated Serum Biomarker Verification and Validation with Wes and Ella   
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Multiplexing with Simple Western   
Simple Western Streamlines Serum Antibody Analysis   
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Breaking Laser Capture Microdissection Sample Size Road Blocks with Simple Western   
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High Molecular Weight Protein Analysis Made Simple   
Total Protein Analysis the Simple Western Way   
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Peggy: size- or charge-based Western blotting at the push of a button   
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Easy Transfer of Your Traditional Western Blot to Wes   
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Detailed Characterization of ERK1 and ERK2 Phosphorylation   
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Screening siRNA and Verifying shRNA Knockouts   
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Brochures and Datasheets

Peggy Sue Datasheet   
Wes Datasheet   
Simple Western Family Brochure   
Sally Sue Datasheet   
Compass Software 21 CFR Part 11 Compliance Tools   
NanoPro 1000 Brochure   

Ebooks

Applications in Bioprocessing    
Advances in Neuroscience   
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Win the race to discovery with Simple Western and Simple Plex.   
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Simplifying Protein Analysis   
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From Your Peers

Wes Gives Researchers Fast Answers at Cancer Research UK, Cambridge Institute   
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Wes Speeds Up the Core at Xavier University of Louisiana   
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Getting to Better Outcomes for Osteoarthritis Patients with Wes at the University of Chester   
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Making the Link Between Obesity and Cancer with the NanoPro 1000 at Roseman University of Health Sciences   
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Wes Takes on Far-Western Blotting to Detect Protein Phosphorylation at UConn Health   
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Wes is Keeping Things Green at the University of Alberta   
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Wes Cuts Time to Results and Solves the Membrane Protein Challenge at the University of Nevada School of Medicine   
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Wes sets a new pace for Alzheimer‘s research at the University of Texas Health Science Center   
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Wes Ups the Data Points Count on Laser Capture Microdissection Samples at East Tennessee State University   
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Wes changes the brain receptor research game at the University of Texas   

Newsletter

Simply Speaking Summer 2016    
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Posters

Maternal Obesity Reduces Skeletal Muscle Insulin Sensitivity in Fetal and Juvenile Japanese Macaques   
Precision Cut Cancer Tissue slices as human model for the testing of immune-modulatory compounds   
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Quantification of Cyclin D1, Cyclin D2, Ikaros, Aiolos and Cereblon Proteins by Capillary Immunoassay in Patients With Newly Diagnosed Multiple Myeloma and Analysis of Their Impact on Patient Survival   
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Analysis of minute amounts of clinical biospecimen for the AXL receptor expression using real-time PCR and Simple Western Size technology.   
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Characterization of a biological active soluble Human Cytomegalovirus gH/gL/pUL128/130/131 pentameric complex   
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Predicting response to Mcl-1 targeting agents in Non-Small Cell Lung Cancer (NSCLC) and Multiple Myeloma (MM) cell lines.   
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Deleterious effects of amyloid beta peptide in the neuromuscular junction: consequences in ALS disease.   
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Development of a IRAK1 Quantitative Protein Degradation Assay for In-vitro and Ex-vivo Target Engagement Using ProteinSimple Capillary Western Technology   
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Toxic events of beta-amyloid oligomers on cortical neurons and protective effect of beta-Estradiol: A mechanistic study (Neuro-Sys, SfN 2014)   
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Eliminating Western Blot Variability for High Molecular Proteins with Sally Sue (HUPO 2014)   
Accurate Quantitation of Proteins Involved In Autoimmune Disease Using Simple Western (AAI & PEGS 2014)   
Accurate Quantitation of Recombinant Proteins with Simple Western (PepTalk 2014)   
Effects of antibody-mediated EGF-receptor inhibition on ERK1/2 isoform phosphorylation in organoid cultures (Indivumed, AACR 2012)   
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Development of a Robust Nanoimmunoassay and Immunohistochemical Assay for ASNS (MD Anderson, AACR 2012)   
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The use of nanoimmunoassay (NIA) technology to predict response to insulin‐like growth factor‐1 receptor (IGF1R) inhibition in head and neck squamous cell carcinoma (HNSCC) (University of Virginia, AACR 2012)   
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Use of Nano-ImmunoAssay to Generate Rapid, Quantitative Nanoscale Proteomic Profiling of the Hypoxia Pathway in Renal Cell Carcinoma Clinical Specimens (Stanford University, ASCO 2012)   
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Size and Charge Based Analysis of ERK1/2 and 4E-BP1 Using Automated Capillary-Based Systems for Nanoscale Protein Analysis   
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A Fully Automated Capillary Electrophoresis System for Western Analysis Using On-Line Stacking as an Easy Tool for the Enhancement of Sensitivity and Resolution   
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Comparison of Conventional Western Blot Analysis with a Fully Automated Capillary Electrophoresis System for Size-based Analysis of the AKT Pathway Signaling Cascade   
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Application of a novel nano-immunoassay platform to assess changes in cIAP1 in response to the SMAC-mimetic, LCL161. Novartis Poster AACR 2011.   
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Assay Development on the NanoPro Platform: 4E-BP1 and 4E-BP2 (SBS 2010)   
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A rapid screening method for monitoring signaling changes in the monocyte cell line U937 (AACR 2009)   
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Application of a nanoimmunoassay platform to assess changes in EGFR-dependent signaling pathways in lung cancer cell line: surgical resections and laser-capture microdissection from patient-derived tumor cells exposed to EGFR tyrosine kinase inhibitors (AACR 2009)   
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Adoption Success: Expansion Of Rapid Screening for Monitoring Signaling Changes (AACR 2009)   
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Independent Measurement of MEK Phosphoforms by Capillary Immunoassay (AACR 2008)   
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Measurement of Oncoproteins in Primary Hematopoietic Malignancies Pre- and Post-Therapy Using a Nano-Immunoassay System (AACR 2008)   
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Measuring Tyrosine Kinase Inhibitor Effects on Cell Signaling Pathways (Beatson 2008)   
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Protein Expression and Cell Signaling Quantified in Rare Cells (ISSCR 2008)   
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Product Inserts

Anti-Human IgG Detection Module for Wes, Peggy Sue, and Sally Sue (DM-005)   
Biotin Detection Module for Wes, Peggy Sue, and Sally Sue (DM-004)   
Total Protein Assay Plate Layout    
No Secondary Detection Module for Wes, Peggy Sue and Sally Sue (DM-003)   
Total Protein Detection Module for Wes, Peggy Sue and Sally Sue (DM-TP01)   
Wes Training Kit (PS-T001)   
Sally Sue or Peggy Sue Size Training Kit (PS-T002)   
Peggy Sue or Sally Sue Separation Module (SM-S001, SM-S002, SM-S003)   
Wes 12-230 kDa 13 Capillary Cartridge Separation Module (SM-W001, SM-W002)   
Wes 12-230 kDa 25 Capillary Cartridge Separation Module (SM-W003, SM-W004)   
Wes 66-440 kDa 13 Capillary Cartridge Separation Module (SM-W005, SM-W006)   
Wes 66-440 kDa 25 Capillary Cartridge Separation Module (SM-W007, SM-W008)   
Wes 2-40 kDa 13 Capillary Cartridge Separation Module (SM-W009, SM-W010)   
Wes 2-40 kDa 25 Capillary Cartridge Separation Module (SM-W011, SM-W012)   
Anti-Rabbit Detection Module for Wes, Peggy Sue and Sally Sue (DM-001)   
Anti-Mouse Detection Module for Wes, Peggy Sue and Sally Sue (DM-002)   
Wes 13-capillary 12-230 kDa Size-based Master Kit with split buffer Product Insert   
Wes 13-capillary 66-440 kDa Size-based Master Kit with split buffer Product Insert   
Wes 13-capillary Total Protein Master Kit with split buffer Product Insert   
Wes 25-capillary 12-230 kDa Size-based Master Kit with split buffer Product Insert   
Wes 25-capillary 66-440 kDa Size-based Master Kit with Split Running Buffer Product Insert   
Wes 25-capillary Total Protein Master Kit with split buffer Product Insert   
Peggy Sue or Sally Sue 12–230 kDa Size-based Master Kit Product Insert    
Peggy Sue, Peggy or NanoPro 1000 XDR Charge Separation Master Kit Product Insert    
Peggy Sue or Sally Sue Total Protein Master Kit Product Insert    
Wes 13-capillary Total Protein Master Kit Product Insert    
Wes 25-capillary Total Protein Master Kit Product Insert    
Peggy Sue or Sally Sue 66–440 kDa Size-based Master Kit Product Insert   
Wes 13-capillary 66–440 kDa Size-based Master Kit Product Insert   
Wes 25-capillary 66–440 kDa Size-based Master Kit Product Insert   
Wes 25-capillary 12-230 kDa Size-based Master Kit Product Insert   
Peggy or Sally Size Master Kit Product Insert    
Simon Master Kit Product Insert   
Peggy or NanoPro XDR Charge Master Kit Product Insert   

Quick Reference Guides

Compass Quick Reference Guide for Simple Western Total Protein Assays   
Compass Quick Reference Guide for Simple Western Size Assays   

Safety Data Sheets

DM-005 SDS Anti-Human IgG Detection Module for Wes, Peggy Sue, and Sally Sue   
DM-004 SDS Biotin Detection Module for Wes, Peggy Sue, and Sally Sue   
043-426 SDS 20X Anti-Rabbit HRP Conjugate   
SM-S001 SDS 12-230 kDa Peggy Sue and Sally Sue Separation Module   
SM-S002 SDS 66-440 kDa Peggy Sue and Sally Sue Separation Module   
SM-S003 SDS 2-40 kDa Peggy Sue and Sally Sue Separation Module   
041-265 SDS Simon Sally Peggy Sample Buffer 400 µL (SDS hazard)   
041-695 SDS Simon Sally Peggy Single Use Fluorescent Standard   
041-808 SDS Antibody Diluent Plus   
042-172 SDS FL Standard with low MW system control   
042-172 SDS French FL Standard with low MW system control   
042-172 SDS German FL Standard with low MW system control   
042-172 SDS Italian FL Standard with low MW system control   
042-174 SDS French Wes Ladder I (12-230 kDa)   
042-174 SDS German Wes Ladder I (12-230 kDa)   
042-174 SDS Italian Wes Ladder I (12-230 kDa)   
042-174 SDS Wes Ladder I (12-230 kDa)   
042-195 SDS 10X Sample Buffer 440 µL (SDS hazard)   
042-203 SDS Antibody Diluent 2 (20 mL)   
042-205 SDS Anti Mouse Secondary Antibody   
042-206 SDS Anti Rabbit Secondary Antibody   
043-379 SDS Peroxide (2.0 mL) Ethanol less than 1.0%   
042-251 SDS DTT 0.4M (40 µL)   
042-251 SDS French DTT 0.4M (40 µL)   
042-251 SDS German DTT 0.4M (40 µL)   
042-251 SDS Italian DTT 0.4M (40 µL)   
042-414 SDS Streptavidin HRP (132 µL)   
042-512 SDS Separation Matrix 2 (12-230 kDa 2.5 mL)   
042-513 SDS Stacking Matrix 2 (1.5 mL)   
042-514 SDS Antibody Diluent 2 (10 mL)   
042-517 SDS Running Buffer 2 (140 mL) hexanol warning   
042-518 SDS Matrix Removal Buffer 2 (140 mL)   
042-518 SDS Matrix Removal Buffer II (140 mL)   
043-311 SDS Luminol S (2 mL)   
042-918 SDS Separation Matrix 3 (2.5 ml)   
042-919 SDS Running Buffer 3 (140 mL) hexanol warning   
042-975 SDS Total Protein Reconstitution Agent 2 (1.8 ml)   
042-976 SDS Total Protein Streptavidin HRP   
043-163 SDS Upper Running Buffer 2 140 mL   
043-164 SDS Lower Running Buffer 2 140 mL   
043-165 SDS Wes 12-230 kDa Pre-filled Plates with Split Buffer   
043-208 SDS Wes 66-440 kDa Pre-filled Plates with Split Buffer   
043-209 SDS Upper Running Buffer 3 Sue 140 mL   
043-210 SDS Lower Running Buffer 3 Sue 140 mL   
043-226 SDS Upper and Lower Running Buffer 3 Kit   
DM-001 SDS Anti Rabbit Detection Module for Wes Peggy Sue and Sally Sue   
DM-002 SDS Anti Mouse Detection Module for Wes Peggy Sue and Sally Sue   
DM-003 SDS No Secondary Detection Module for Wes Peggy Sue and Sally Sue   
DM-TP01 SDS Total Protein Detection Module for Wes Peggy Sue and Sally Sue   
PS-FL01-8 SDS Fluorescent 5X Master Mix 1   
PS-FL01 SDS Fluorescent 5X Master Mix 1   
PS-FL03-8 SDS Fluorescent 5X Master Mix 3   
PS-FL03 SDS Fluorescent 5X Master Mix 3   
PS-FL05-8 SDS Fluorescent 5X Master Mix 5   
PS-FL05 SDS Fluorescent 5X Master Mix 5   
PS-MK01 SDS Wes Rabbit (12-230 kDa) Master Kit 200 data points   
PS-MK02 SDS Wes Mouse (12-230 kDa) Master Kit 200 data points   
PS-MK04 SDS Wes Rabbit Master Kit (12-230 kDa) 104 data points   
PS-MK05 SDS Wes Mouse Master Kit (12-230 kDa) 104 data points   
PS-MK06 SDS Sally Sue or Peggy Sue Rabbit (12-230 kDa) Master Kit   
PS-MK07 SDS Sally Sue or Peggy Sue Mouse (12-230 kDa) Master Kit   
PS-MK08 SDS Wes Rabbit (66-440 kDa) Master Kit   
PS-MK09 SDS Wes Mouse (66-440 kDa) Master Kit   
PS-MK10 SDS Wes 66-440 kDa Rabbit Master kit 104 data points   
PS-MK11 SDS Wes 66-440 kDa Mouse Master kit 104 data points   
PS-MK12 SDS Peggy Sue or Sally Sue 66-440 kDa Rabbit Size Master Kit   
PS-MK13 SDS Peggy Sue or Sally Sue 66-440 kDa Mouse Size Master Kit   
PS-MK14 SDS Wes Rabbit (12-230 kDa) Master Kit with split buffer 200 data points   
PS-MK15 SDS Wes Mouse (12-230 kDa) Master Kit with split buffer 200 data points   
PS-MK16 SDS Wes 12-230 kDa Rabbit Master kit with Split Running Buffer 104 data points   
PS-MK17 SDS Wes 12-230 kDa Mouse Master kit with Split Running Buffer 104 data points   
PS-MK18 SDS Peggy Sue or Sally Sue Rabbit (12-230 kDa) Size Separation Master Kit with Split Buffer   
PS-MK19 SDS Peggy Sue or Sally Sue Mouse (12-230 kDa) Size Separation Master Kit with Split Buffer   
PS-MK20 SDS Wes Rabbit (66-440 kDa) Master Kit with split buffer 200 data points   
PS-MK21 SDS Wes Mouse (66-440 kDa) Master Kit with split buffer 200 data points   
PS-MK22 SDS Wes 66-440 kDa Rabbit Master kit with Split Running Buffer 104 data points   
PS-MK23 SDS Wes 66-440 kDa Mouse Master kit with Split Running Buffer 104 data points   
PS-MK24 SDS Peggy Sue or Sally Sue Rabbit (66-440 kDa) Size Separation Master Kit with Split Buffer   
PS-MK25 SDS Peggy Sue or Sally Sue Mouse (66-440 kDa) Size Separation Master Kit with Split Buffer   
PS-ST01 SDS Standard Pack 1 (12-230 kDa)   
PS-ST02 SDS Standard Pack 2   
PS-ST03 SDS Standard Pack 3   
PS-TP05 SDS Peggy Sue or Sally Sue 12-230 kDa Total Protein Master Kit   
PS-TP06 SDS Peggy Sue or Sally Sue 66-440 kDa Total Protein Master Kit   
PS-TP07 SDS Wes 12-230 kDa Total Protein Master kit with Split Buffer 200 data points   
PS-TP08 SDS Wes 66-440 kDa Total Protein Master kit with Split Buffer 200 data points   
PS-TP09 SDS Wes 12-230 kDa Total Protein Master kit with Split Buffer 104 data points   
PS-TP10 SDS Wes 66-440 kDa Total Protein Master kit with Split Buffer 104 data points   
PS-TP11 SDS Peggy Sue or Sally Sue 12-230 kDa Total Protein Master Kit with Split Buffer   
PS-TP12 SDS Peggy Sue or Sally Sue 66-440 kDa Total Protein Master Kit with Split Buffer   
SM-W001 SDS 12-230 kDa Wes Separation Module   
SM-W002 SDS 12-230 kDa Wes Separation Module   
SM-W003 SDS 12-230 kDa Wes Separation Module   
SM-W004 SDS 12-230 kDa Wes Separation Module   
SM-W005 SDS 66-440 kDa Wes Separation Module   
SM-W006 SDS 66-440 kDa Wes Separation Module   
SM-W007 SDS 66-440 kDa Wes Separation Module   
SM-W008 SDS 66-440 kDa Wes Separation Module   
SM-W009 SDS 2-40 kDa Wes Separation Module   
SM-W010 SDS 2-40 kDa Wes Separation Module   
SM-W011 SDS 2-40 kDa Wes Separation Module   
SM-W012 SDS 2-40 kDa Wes Separation Module   
040-649 SDS Sample Diluent   
042-520 SDS Wash Buffer (170 ml)   
042-521 SDS Luminol (2.0 mL)   
042-522 SDS Peroxide (2.0 mL) Ethanol less than 1.0%   
042-202 SDS Wash Buffer (60 mL)   
042-233 SDS Luminol S   
042-234 SDS Peroxide (1.5 mL)   
CBS201S1 SDS Simon Size Separation Kit   
PS-PP01 SDS 12-230 kDa Pre-filled plates (8)   
PS-PP02 SDS 66-440 kDa Pre-filled plates (8)   
PS-PP03 SDS Wes 12-230 Pre-filled Plates with Split Buffer   
PS-PP04 SDS 66-440 kDa Pre-filled plates with Split Running Buffer (8)   
040-024 SDS pI Standard 4.0   
040-025 SDS pI Standard 4.2   
040-026 SDS pI Standard 4.4   
040-027 SDS pI Standard 4.9   
040-028 SDS pI Standard 5.5   
040-029 SDS pI Standard 6.0   
040-031 SDS pI Standard 7.0   
040-032 SDS pI Standard 7.3   
040-337 SDS Anolyte refill   
040-338 SDS Catholyte refill   
040-482 SDS Aqueous Inhibitor Mix contains Sodium Fluoride more than 0.1% and less than 5%   
040-510 SDS DMSO Inhibitors Dimethylsulfoxide (DMSO)   
040-644 SDS pI Standard Ladder 1   
040-646 SDS pI Standard Ladder 3   
040-764 SDS Bicine CHAPS Lysis Buffer and Sample Diluent   
040-783 SDS Bicine CHAPS Cell Wash Buffer   
040-790 SDS pI Standard 9.7   
041-108 SDS Wash Concentrate Refill for Peggy or NanoPro 1000   
CBS2001 SDS XDR Charge Separation Master Kit for Peggy Sue Peggy or NanoPro 1000   
040-645 SDS pI Standard Ladder 2   

Scientific Articles

Isoelectric focusing technology quantifies protein signaling in 25 cells   
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Specification Sheets

Simple Western Low Molecular Weight Assays Spec Sheet   

Technical Notes

How To Avoid Cross-Reactivity with the 230 kDa Fluorescent Standard   
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Molecular Weight Determination by Electrophoresis of SDS-Denatured Proteins   
[show details] 
Simple Western Delivers Improved Serum Biomarker Sensitivity with No Secondary IgG Cross-Reactivity   
[show details] 
Get More Data Out of Precious Samples with Simple Western   
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Improved Multiplexing with Simple Western using a 20X Rabbit HRP Conjugate   
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Superior Control of Simple Western Size Assays with a Dynamic Range Improvement   
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Wes Delivers Broader Dynamic Range Compared to Traditional Western   
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User Guides

Compass Quick Reference Guide for Simple Western Total Protein Assays   
Simple Western Size Assay Buffer Compatibility   
Compass Quick Reference Guide for Simple Western Size Assays   
Wes Installation Guide   
Wes User Guide   
Compass for Simple Western Software User Guide for Wes, Peggy Sue and Sally Sue   
Sally Sue and Peggy Sue User Guide   
Simple Western Size Troubleshooting Guide   
Simple Western Size Assay Development Flowchart   
Simple Western Size Assay Development Guide   
Compass for NanoPro 1000 User Guide   
Compass for Simon User Guide   
NanoPro 1000 User Guide   
Peggy User Guide   
Sally User Guide   
Simon User Guide   
NanoPro Assay Development User Guide