| | Simple Westernでマルチプレックス | Japanese | [概要表示] |
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この「how to」ガイドでは、Simple Westernで迅速かつ効率的にマルチプレックスアッセイを行う方法を説明し、高発現タンパク質と低発現タンパク質を同時に観察する方法についていくつかのコツを紹介します。 |
| | Lentiviral Vector Analysis for Cell and Gene Therapy Made Simple | [概要表示] |
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Simple Western is a capillary electrophoresis immunodetection platform that allows you to measure lentiviral vector identity, titer, stability, purity, capsid content, and transduction efficiency, all in one instrument. |
| | AAV2 VP Protein Standards and Their Use in Quantifying Capsid Protein Ratio by Western Blot and Simple Western | [概要表示] |
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Comprehensive monitoring of VP1, VP2, and VP3 protein concentrations and their molar ratios is an effective tool to ensure initial optimization and consistent quality of the final AAV gene therapy products. Here we introduce PROGEN´s recombinant VP standards used with Simple Western™ automated capillary electrophoresis in fit-for-purpose solutions for VP capsid protein ratio and viral protein titer measurements. We further show the establishment of the AAV2 VP protein standards by PROGEN as well as the assay performance data that demonstrates linearity and high sensitivity in an automated workflow optimized for AAV samples. |
| | Do Your AAVs Contain DNA? Rapid and Sensitive Empty/Full Capsid Quantification with Simple Western | [概要表示] |
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In this Application Note, we developed a novel method for quantifying the DNA content of AAV particles with Simple Western™, a next-generation biomolecular analytical tool that seamlessly combines capillary electrophoresis and immunodetection with conventional Western blot antibodies. Here, the Simple Western method automatically separates AAV samples by Size or Charge followed by specific and sensitive detection using anti-DNA and anti-VP1/2/3 antibodies directly in the capillary for quantitative and reproducible measurement of these central AAV components. For each antibody, we identify the range in which the signal intensity is linearly related to the amount of sample loaded, resulting in a rapid and sensitive assay for accurately quantifying the ratio of % full AAVs to total AAVs in a sample (also known as the Content Ratio). |
| | Shining New Light on Pharmacokinetic Assays with Simple Western | [概要表示] |
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Simple Western was used to separate proteins by charge and size to evaluate the pharmacokinetic properties of adalimumab and two adalimumab biosimilars in human serum. |
| | Keeping the Promise of Immuno-Oncology with Simple Western and Single-Cell Western | [概要表示] |
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Simple Western and Single-Cell Western have the potential to make significant contributions to three important branches of immuno-oncology: angiogenesis of the tumor microenvironment, immune checkpoint inhibition, and chimeric antigen T-cell (CAR T-cell) therapy. Learn more. |
| | Simple Westernでバイオプロセス中の不純物質の検出 -医薬品製造過程関連の不純物の同定および定量- | Japanese | [概要表示] |
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このアプリケーションノートでは、バイオ医薬品およびワクチン開発プロセスの様々な段階で存在し得る4つの主要なコンタミ物質(ホストセル蛋白質(HCP)、Protein A、GFP(緑色蛍光蛋白質)およびBSA(ウシ血清アルブミン))の正確な検出を中心に説明します。 |
| | Bioprocess Contaminant Detection using Simple Western | [概要表示] |
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In this application note, we focus on the accurate detection of four candidate contaminants that may be present during various stages of the therapeutic protein and vaccine development processes: host cell protein (HCP), Protein A, green fluorescence protein (GFP) and bovine serum albumin (BSA). |
| | Multiplexing with Simple Western | |
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| | Breaking Laser Capture Microdissection Sample Size Road Blocks with Simple Western | [概要表示] |
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Laser capture microdissection (LCM) is a powerful tool to identify and isolate a pure sample of the cell type you're
interested in. But, proteomic studies with LCM samples are really restricted by the small amounts of tissue collected with
each capture since there just isn't much to work with. You often have to use the entire sample captured for traditional
Western blot analysis and that only leaves you with one data point! So, researchers often use 2D Electrophoresis and
mass spectrometry instead to max out the amount of data they can generate. Both of these methods have their own
limitations when it comes to ease-of-use and reproducibility though. Immunohistochemistry is also used to analyze LCM
samples as it's a more accessible technique, but it really doesn't give you a lot of info either.
Simple Western is an automated capillary-based immunoassay that changes the proteomic research game. You only
need 1-10 µL of LCM sample for each data point, so you'll get more data points for each sample you collect. Not to
mention the sensitivity that comes with it will even let you analyze proteins you couldn't previously do with traditional
Western blot. And it's all wrapped up in a simple workflow that minimizes your hands-on time. Simple Western is a
sensitive, easy-to-use analytical tool that ups the ante on protein analysis with LCM samples.
In this application note, we'll show you two examples of how Simple Western changed what researchers could do with
their precious LCM samples for the better |
| | Screening siRNA and Verifying shRNA Knockouts | [概要表示] |
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RNA interference (RNAi) is an RNA-dependent gene silencing mechanism that can affect the expression of specific genes by inhibiting translation or suppressing transcription epigenetically. Using Firefly assays, RNAi effects such as impact on phosphorylation or silencing can be studied functionally in samples as small as 100 cells. An additional benefit of the small samples size is that a variety of conditions can be studies in a single assay. |
| | Detailed Characterization of ERK1 and ERK2 Phosphorylation | [概要表示] |
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Monophospho- and diphospho-ERK isoforms are not resolved by traditional Western blot analysis, and the sample quantity required is relatively large. The Firefly ERK1/ERK2 assay can distinguish and quantify unphosphorylated, mono and dual-phosphorylated isoforms of ERK1 and ERK2, allowing a more accurate determination of ERK activation than Western blots provide. |