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Simple Plex assay for the detection of human Cysteine-rich
angiogenic inducer 61 (Cyr61) in serum and plasma (EDTA/Heparin)
Protein analysis plays an important role in stem cell research, and for most researchers, Western blotting is a key component of their toolbox. From verifying pluripotency and identifying lineage-specific cell types to identifying key modulators of cell signaling pathways, to evaluating disease models and therapeutic approaches, the right protein analysis
techniques can help you achieve success. In this eBook, we examine technological advances in Western blotting and protein analysis that are at the forefront of stem cell research. Learn how automated Western systems and Single-Cell Westerns can advance your stem cell research.
Simple Plex assay for the detection of human Interleukin 22
(IL-22) in cell culture supernatant (CCS), serum, and plasma
In drug discovery, confirmation of in-cell target engagement is a critical component of the drug development process. Confirming that a drug candidate engages its proposed target in the cell and determining the concentration at which it exerts the desired effect(s) fulfill fundamental criteria for translation to activity and efficacy in its target tissue. Thermal shift assays (TSA) are regularly used by industry and academia to uncover or confirm interactions using purified proteins. Recently, this type of assay has been adapted to a cellular format and is called the cellular thermal shift assay (CETSA). In this application note, quantitative and reproducible CETSA data generated with ProteinSimple’s Wes instrument (CETSA-Wes) are presented. This assay verifies drug target binding, and given its quantitative nature, the half maximal inhibitory concentration (IC50) is also calculated.
Designed to eliminate limitations of existing immunoassay platforms, Simple Plex assays utilize a low volume microfluidic cartridge format and are run in automated fashion on the Ella instrument, enabling sensitive measurement of up to 4 analytes in about an hour.
Here we describe recent external immuno-oncology studies evaluating Simple Plex assay performance for biomarker measurements from a range of sample types. Dynamic range, sensitivity, and reproducibility were all important test criteria in these studies evaluating dysregulation of pro-inflammatory cytokines and expression of immune checkpoint targets and downstream mediators.
Results demonstrated consistent sensitivity at low picogram/mL levels with 10% CV or less, a 4-5 log dynamic range across all sample types, and significant time savings versus traditional ELISA. Overall, Simple Plex assays enabled more sensitive detection, in a quarter of the time, from a quarter of the sample volume for measuring single or multiple analytes.
ProteinSimple iCE CFR software is used for system control, data acquisition and collection of critical audit information. The files used by the iCE CFR software are created using a file format unique to the software. The file types created by the iCE CFR software have the extensions ICF, CAL, DAT, BCH, IEF and ITF. This document provides file type descriptions and file locations for iCE CFR software V3 and V4. Recommendations for data backup are also provided.
SDS for Control Concentrate Human CXCL9/MIG. Catalog number 894951
SDS for Catalog number 894950, Control Concentrate Human CXCL8/IL-8
catalog number 894949, Control Concentrate Human CXCL10/IP-10
894952, Control Concentrate Human EGF